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RAS PhysiologyЖурнал эволюционной биохимии и физиологии Journal of Evolutionary Biochemistry and Physiology

  • ISSN (Print) 0044-4529
  • ISSN (Online) 3034-5529

STUDY OF THE FORMATION OF PROTEIN-DUST AGGREGATES IN A SOLUTION OF SERUM ALBUMIN CONTAINING DUST FROM A MINING AND METALLURGICAL ENTERPRISE

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PII
S3034552925040037-1
DOI
10.7868/S3034552925040037
Publication type
Article
Status
Published
Authors
T.N. Shtin
  • Affiliation: Yekaterinburg Medical Research Center for Prophylaxis and Health Protection in Industrial Workers
I.A. Kholmanskikh
  • Affiliation: Yekaterinburg Medical Research Center for Prophylaxis and Health Protection in Industrial Workers
S.A. Shtin
  • Affiliation: Federal State Autonomous Educational Institution of Higher Education "Ural Federal University named after the first President of Russia B.N. Yeltsin"
Volume/ Edition
Volume 61 / Issue number 4
Pages
241-250
Abstract
Nanoparticles contained in airborne aerosols are a significant risk factor and require a comprehensive approach to studying and eliminating the gap in knowledge about interaction with biological media. The aim of the work is to study the dynamics of the interaction of industrial dust with serum proteins and to develop a new, integrated approach to assessing exposure and the impact of environmental components on health. The paper shows the results of studies of the interaction of isolated fractions of industrial dust with a solution of serum albumin using diffraction grating and molecular absorption spectroscopy, and its elemental composition is determined by the ICP-MS method. It was found that after 24 hours of exposure to industrial dust in a protein solution, protein-dust particles agglomerate, followed by their decomposition by 744 hours and the particle size approaches the initial one. An inverse correlation was found between the specific surface area of the particles, their diffusion coefficient and size. The results obtained by the diffraction grating method are in good agreement with the qualitative characteristics obtained by molecular absorption spectroscopy. The ICP-MS method has recorded the complex dynamics of the redistribution of elements between phases over time. An assumption is made about the interaction of the analyzed elements with the protein, leading to the formation of nanoscale aggregates and the binding of metals, which is important for understanding their biological effects. Experimental data can be used to determine the standards of air aerosol pollution.
Keywords
наночастица белок коэффициент диффузии удельная поверхность индуцированная решетка пыль горная металлургия
Date of publication
22.08.2025
Year of publication
2025
Number of purchasers
0
Views
26

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