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RAS PhysiologyЖурнал эволюционной биохимии и физиологии Journal of Evolutionary Biochemistry and Physiology

  • ISSN (Print) 0044-4529
  • ISSN (Online) 3034-5529

Mitochondrial dynamics and metabolic remodeling in xenograft of IPSC-derived human neural precursors

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PII
10.31857/S0044452924030107-1
DOI
10.31857/S0044452924030107
Publication type
Article
Status
Published
Authors
D. N Voronkov
  • Affiliation:
    Research Center of Neurology
    N.I. Pirogov Russian National Research Mediвcal University
Volume/ Edition
Volume 60 / Issue number 3
Pages
320-328
Abstract
It is well recognized that the regulation of mitochondrial functions affects the differentiation and maturation of neurons. The study of these processes is of both fundamental and practical importance for regenerative neurobiology. Aim of the study: to characterize the mitochondrial fission changes and their relation to the activation of oxidative phosphorylation (metabolic shift) during maturation of human IPSC-derived neural precursors grafted into rat striatum. Wistar rats (n = 15) were unilaterally injected into the caudate nucleus with neural precursors derived from human IPSCs. Changes in localization and expression of neuronal differentiation markers: nestin, NeuN, neuronal enolase, as well as mitochondrial outer membrane protein, ATP synthase and mitochondrial fission protein Drp1 were assessed by immunostaining. Measurements were performed on graft cells 2 weeks, 3 and 6 months after surgery. Maturation of grafted neurons was associated with fluctuations morphometric parameters of the mitochondrial fraction and Drp1 levels. Increased mitochondrial fission was detected 3 months after transplantation, before an increase in ATP synthase staining by 6th month and a switch of transplanted cells to oxidative phosphorylation. The conducted experiment demonstrated a link between mitochondrial dynamics and changes in the metabolic profile and maturation of transplanted neurons. The regulation of mitochondrial dynamics may have future implications for developing methods to improve the integration of transplanted neurons into recepient brain structures.
Keywords
нейрогенез трансплантация индуцированные плюрипотентные клетки человека митохондриальная динамика Drp1 окислительное фосфорилирование
Date of publication
15.05.2024
Year of publication
2024
Number of purchasers
0
Views
11

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