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RAS PhysiologyЖурнал эволюционной биохимии и физиологии Journal of Evolutionary Biochemistry and Physiology

  • ISSN (Print) 0044-4529
  • ISSN (Online) 3034-5529

Effect of adrenaline on mitochondrial membrane potential and indicators of the cellular immunity of hemocytes of the Mediterranean mussel (Mytilus galloprovincialis)

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PII
10.31857/S0044452924070042-1
DOI
10.31857/S0044452924070042
Publication type
Article
Status
Published
Authors
A. A. Tkachuk
  • Affiliation:
    A.O. Kovalevsky Institute of Biology of the Southern Seas of RAS
    Sechenov Institute of Evolutionary Physiology and Biochemistry of the Russian Academy of Sciences
Volume/ Edition
Volume 60 / Issue number 6
Pages
617-625
Abstract
Bivalves as inhabitants of the littoral zone of the World Ocean are subjected to fluctuations in abiotic environmental factors. Sharp fluctuations in environmental parameters are accompanied by the development of a physiological stress reaction in the organism of mollusks, while changes in their functional state occur due to the release of neurotransmitters into the hemolymph. Catecholamines are key signaling molecules in the system of neuroendocrine regulation of bivalve mollusks and also are involved in the modulation of the immune response during physiological stress. Hemocytes, as the central effector of the cellular immunity of bivalve mollusks, have adrenoreceptors on the surface of the cell membrane, which suggests the presence of a functional relationship between external stress and the cellular immune response. In the present work, the effect of adrenaline at concentrations of 1 and 10 μM on phagocytosis, adhesion and aggregation capacity of hemocytes of the Mediterranean mussel Mytilus galloprovincialis (Lamarck, 1819) was investigated in vitro. The effect of adrenaline on the level of spontaneous production of reactive oxygen species and on changes in the mitochondrial membrane potential of hemocytes was also studied. It was shown that stimulation of mussel hemocytes with adrenaline at a concentration of 10 μM contributed to a reliable increase in the ability to phagocytosis. Adrenaline at a concentration of 1 μM significantly increased the ability of hemocytes to adhere to a solid substrate. Also, stimulation of cells with adrenaline at 10 μM for 30 minutes led to an increase in the membrane potential of hemocyte mitochondria. No reliable changes in the level of spontaneous production of active forms of oxygen in hemocytes under the influence of adrenaline were detected. The results of this work indicate that adrenaline has an immunomodulatory effect on mussel hemocytes and stimulates their aerobic metabolism.
Keywords
средиземноморская мидия гемоциты адреналин фагоцитоз адгезия активные формы кислорода мембранный потенциал митохондрий
Date of publication
15.11.2024
Year of publication
2024
Number of purchasers
0
Views
13

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