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RAS PhysiologyЖурнал эволюционной биохимии и физиологии Journal of Evolutionary Biochemistry and Physiology

  • ISSN (Print) 0044-4529
  • ISSN (Online) 3034-5529

The Effects of Phenanthrene on Electrical Activity in Ventricular Cardiomyocytes of Atlantic Cod (Gadus morhua)

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PII
10.31857/S0044452924040043-1
DOI
10.31857/S0044452924040043
Publication type
Article
Status
Published
Authors
T. S. Filatova
  • Affiliation: Department of Human and Animal Physiology, Lomonosov Moscow State University
Volume/ Edition
Volume 60 / Issue number 4
Pages
372-382
Abstract
The production of oil on the Arctic shelf and its transport along the Northern Sea Route increase risks of pollution of the ecosystems in the Arctic seas with oil and oil products. Today, polyaromatic hydrocarbons are known as the most toxic oil components, and phenanthrene is predominant in terms of its concentration in oil and physiological effects. Phenanthrene affects the electrical activity of fish heart, but its effects are species-specific. At the same time, the effects of phenanthrene on cardiac function in Arctic fishes, including economically important commercial species, are studied not enough. This study examines the effects of phenanthrene on electrical activity and ionic currents in ventricular myocardium of Atlantic cod (Gadus morhua). The major ionic currents in cod myocardium were IKr, IK1, INa and ICa. Phenanthrene (1 μM) did not affect the duration of action potentials (APs) recorded in isolated cod ventricular cardiomyocytes using patch clamp method. Meanwhile, phenanthrene suppressed rapid delayed rectifier current IKr by 61.33 ± 3.94%, decreasing the repolarization reserve of the myocardium. Phenanthrene did not affect nor the level of resting membrane potential, not background inward rectifier current IK1. Also, application of phenanthrene decreased AP upstroke velocity in cod myocytes, which was due to the suppression of fast sodium current INa. Finally, phenanthrene slightly reduced the amplitude of calcium current ICa and accelerated its inactivation, which overall led to the decrease in ICa charge transfer. Thus, the effects of phenanthrene on cod myocardium at cellular level can be described as potentially proarrhythmic, which makes the populations of cod in Arctic seas vulnerable to pollution of the aquatic environment by oil components after oil spills due to technological disasters.
Keywords
сердце рыба полиароматические углеводороды Арктика потенциал действия ионные токи кардиотоксичность
Date of publication
15.07.2024
Year of publication
2024
Number of purchasers
0
Views
12

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